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99
ATCC 2021 n a bhk 21 wi 2 whitt
2021 N A Bhk 21 Wi 2 Whitt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2021 n a bhk 21 wi 2 whitt - by Bioz Stars, 2026-10
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92
Addgene inc il21r
Figure 1. IL-21/IL-21R signaling reduces murine L-GMPs in vivo (A) <t>Il21R</t> mRNA expression (qRT-PCR) in FACS-sorted L-GMPs from the BM of BL/6 AML mice 35 days after leukemia transplantation (n = 10 mice). Red bar indicates the mean. Each dot represents the mean of two technical replicates. (B) IL-21 levels in BM samples from AML mice (n = 11 mice) and naive controls (n = 5 mice). Each dot represents the mean of two technical replicates. Data are shown as mean ± SEM. Statistics: Student’s t test. (C) Fold change colony-forming units from FACS-sorted L-GMPs cultured in methylcellulose for 7 days in the presence or absence of 300 pg/mL rm-IL-21 (n = 6 mice/group). Each dot represents the mean of two technical replicates. Pooled data from two independent experiments are shown and displayed as mean ± SD. Statistics: Student’s t test. (D) Experimental setup: 5 3 104 MLL-AF9-GFP-transduced LSKs from the BM of BL/6 donors were injected intravenously into non-irradiated BL/6 and Il21/
Il21r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stream+2%2E1+image+analysis+software/LIIalpha+F+4-5+(Plasmid+%2354352)/pm39536753-222-14-31
Average 92 stars, based on 1 article reviews
il21r - by Bioz Stars, 2026-10
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Image Search Results


Figure 1. IL-21/IL-21R signaling reduces murine L-GMPs in vivo (A) Il21R mRNA expression (qRT-PCR) in FACS-sorted L-GMPs from the BM of BL/6 AML mice 35 days after leukemia transplantation (n = 10 mice). Red bar indicates the mean. Each dot represents the mean of two technical replicates. (B) IL-21 levels in BM samples from AML mice (n = 11 mice) and naive controls (n = 5 mice). Each dot represents the mean of two technical replicates. Data are shown as mean ± SEM. Statistics: Student’s t test. (C) Fold change colony-forming units from FACS-sorted L-GMPs cultured in methylcellulose for 7 days in the presence or absence of 300 pg/mL rm-IL-21 (n = 6 mice/group). Each dot represents the mean of two technical replicates. Pooled data from two independent experiments are shown and displayed as mean ± SD. Statistics: Student’s t test. (D) Experimental setup: 5 3 104 MLL-AF9-GFP-transduced LSKs from the BM of BL/6 donors were injected intravenously into non-irradiated BL/6 and Il21/

Journal: Cell reports. Medicine

Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.

doi: 10.1016/j.xcrm.2024.101826

Figure Lengend Snippet: Figure 1. IL-21/IL-21R signaling reduces murine L-GMPs in vivo (A) Il21R mRNA expression (qRT-PCR) in FACS-sorted L-GMPs from the BM of BL/6 AML mice 35 days after leukemia transplantation (n = 10 mice). Red bar indicates the mean. Each dot represents the mean of two technical replicates. (B) IL-21 levels in BM samples from AML mice (n = 11 mice) and naive controls (n = 5 mice). Each dot represents the mean of two technical replicates. Data are shown as mean ± SEM. Statistics: Student’s t test. (C) Fold change colony-forming units from FACS-sorted L-GMPs cultured in methylcellulose for 7 days in the presence or absence of 300 pg/mL rm-IL-21 (n = 6 mice/group). Each dot represents the mean of two technical replicates. Pooled data from two independent experiments are shown and displayed as mean ± SD. Statistics: Student’s t test. (D) Experimental setup: 5 3 104 MLL-AF9-GFP-transduced LSKs from the BM of BL/6 donors were injected intravenously into non-irradiated BL/6 and Il21/

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

Techniques: In Vivo, Expressing, Quantitative RT-PCR, Transplantation Assay, Cell Culture, Injection, Irradiation