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2021 n a bhk 21 wi 2 whitt 2021 N A Bhk 21 Wi 2 Whitt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/stream+2%2E1+image+analysis+software/BHK-21/pm35988541-95-66-74 Average 99 stars, based on 1 article reviews
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Journal: Cell reports. Medicine
Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
doi: 10.1016/j.xcrm.2024.101826
Figure Lengend Snippet: Figure 1. IL-21/IL-21R signaling reduces murine L-GMPs in vivo (A) Il21R mRNA expression (qRT-PCR) in FACS-sorted L-GMPs from the BM of BL/6 AML mice 35 days after leukemia transplantation (n = 10 mice). Red bar indicates the mean. Each dot represents the mean of two technical replicates. (B) IL-21 levels in BM samples from AML mice (n = 11 mice) and naive controls (n = 5 mice). Each dot represents the mean of two technical replicates. Data are shown as mean ± SEM. Statistics: Student’s t test. (C) Fold change colony-forming units from FACS-sorted L-GMPs cultured in methylcellulose for 7 days in the presence or absence of 300 pg/mL rm-IL-21 (n = 6 mice/group). Each dot represents the mean of two technical replicates. Pooled data from two independent experiments are shown and displayed as mean ± SD. Statistics: Student’s t test. (D) Experimental setup: 5 3 104 MLL-AF9-GFP-transduced LSKs from the BM of BL/6 donors were injected intravenously into non-irradiated BL/6 and Il21/
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467
Techniques: In Vivo, Expressing, Quantitative RT-PCR, Transplantation Assay, Cell Culture, Injection, Irradiation